inos-specific inhibitor n-[3-(aminomethyl)benzyl]acetamidine (1400w)  (Cayman Chemical)

Journal: bioRxiv

Article Title: Genetic Manipulation of Caveolin-1 in the Mouse Model of Marfan Syndrome Associated Aortic Root Aneurysm: Effects on Endothelial and Smooth Muscle Function

doi: 10.1101/2024.07.29.605631

Figure Lengend Snippet: Presented bargraphs showcase the impact of 1400W, a potent inhibitor of inducible NOS (iNOS) on PE-induced contraction in aortic rings isolated from male and female MFS/ Cav1KO mice. Data clearly shows that pre-treatment of aortic rings with 1400W (1 µM) has no effects on PE- induced aortic contraction in age-matched male and female mice, indicating that the excessive increase in aortic endogenous NO production in the absence of Cav1 is not mediated by iNOS activation (Means ± SE, N = 6-8 mic/group, Two Way ANOVA followed by Tukey’s pairwise comparison, P ≤ 0.05).

Article Snippet: All pharmacological agonists and blockers including vasoconstricting agent phenylephrine (PE), vasodilatory agent acetylcholine (ACh), non-specific and reversible nitric oxide synthase (NOS) inhibitor L-N G -Nitro arginine methyl ester (L-NAME), and specific and potent inhibitor of inducible nitic oxide synthase (iNOS) 1400W dihydrochloride, were purchased from Sigma Millipore (Sigma Millipore, St. Louis, MO).

Techniques: Isolation, Activation Assay, Comparison

Journal: Photochemistry and photobiology

Article Title: Antagonistic Effects of Endogenous Nitric Oxide in a Glioblastoma Photodynamic Therapy Model

doi: 10.1111/php.12636

Figure Lengend Snippet: Viability loss of glioblastoma cells after a photodynamic challenge. (a) ALA-treated U87 cells were exposed to the indicated light fluences in the absence (○) or presence of 25 μM 1400W (□) or 25 μM cPTIO (△). After irradiation, cells were switched to serum-containing medium, and after 20 h of dark incubation, examined for viability by MTT assay. (b) U251 cells were sensitized identically to U87 cells, irradiated in the absence (○) vs. presence of 25 μM 1400W (□) or 1 mM L-NAME (△), and then examined for viability after 20 h in the dark. Also represented in (a) and (b) are ALA-only or light-only controls (x). Plotted values in (a) and (b) are means ± SEM (n=3); W, cP, and N denote 1400W, cPTIO, and L-NAME, respectively.

Article Snippet: Chemicals, Reagents, and Antibodies The non-specific NOS inhibitor L-N G -nitroarginine methyl ester (L-NAME), iNOS-specific inhibitor N-[3-(aminomethyl)benzyl]acetamidine (1400W), NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), and NO fluorophore probe 4,5-diaminofluorescein diacetate (DAF-2DA) were obtained from Cayman Chemicals (Ann Arbor, MI).

Techniques: Irradiation, Incubation, MTT Assay

Journal: Photochemistry and photobiology

Article Title: Antagonistic Effects of Endogenous Nitric Oxide in a Glioblastoma Photodynamic Therapy Model

doi: 10.1111/php.12636

Figure Lengend Snippet: Apoptotic photokilling of U87 cells and effects of NOS inhibitors. U87 cells at 40–45% confluence were treated with 1 mM ALA without or with a NOS inhibitor (25 μM 1400W or 1 mM L-NAME), and then irradiated (1 J/cm2). After 4 h of follow-up dark incubation, the cells were treated with Annexin V-FITC and Propidium Iodide (PI) according to supplier recommendations. Camptothecin (CPT, 25 μM) served as a maximum apoptosis standard. Extent of staining was quantified using a plate reater with excitation/emission wavelengths of 485/535 nm for Annexin V-FITC and 560/595 nm for PI. Less than 5% PI staining was detected for any of the treatment conditions.

Article Snippet: Chemicals, Reagents, and Antibodies The non-specific NOS inhibitor L-N G -nitroarginine methyl ester (L-NAME), iNOS-specific inhibitor N-[3-(aminomethyl)benzyl]acetamidine (1400W), NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), and NO fluorophore probe 4,5-diaminofluorescein diacetate (DAF-2DA) were obtained from Cayman Chemicals (Ann Arbor, MI).

Techniques: Irradiation, Incubation, Staining

Journal: Photochemistry and photobiology

Article Title: Antagonistic Effects of Endogenous Nitric Oxide in a Glioblastoma Photodynamic Therapy Model

doi: 10.1111/php.12636

Figure Lengend Snippet: Images of NO accumulation in U87 cells after a photodynamic challenge. ALA-treated U87 cells were irradiated in the absence vs. presence of a NOS inhibitor as specified in Fig. 2. An ALA-only dark control without or with L-NAME (1 mM) was included. At the indicated post-irradiation times, the cells were treated with DAF-2DA (20 μM) for 20 min in the dark, and then examined by fluorescence microscopy using 488 nm excitation and 650 nm emission. Image intensities were quantified using ImageJ software. Means ± SEM of values from three separate experiments are shown.

Article Snippet: Chemicals, Reagents, and Antibodies The non-specific NOS inhibitor L-N G -nitroarginine methyl ester (L-NAME), iNOS-specific inhibitor N-[3-(aminomethyl)benzyl]acetamidine (1400W), NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), and NO fluorophore probe 4,5-diaminofluorescein diacetate (DAF-2DA) were obtained from Cayman Chemicals (Ann Arbor, MI).

Techniques: Irradiation, Control, Fluorescence, Microscopy, Software